The Department of Bacteriology at UW-Madison is equipped with a wide range of advanced analytical instruments, including LC–MS/MS, HPLC, Flash chromatography, Rotavapor, TLCs and Solid phase extraction (SPE) assembly purification systems, supporting comprehensive research in biochemistry, metabolomics, and natural product chemistry.
Mass Spectrometry (MS): Orbitrap Exploris 240 System
o Quadrupole, ion routing multipole, and Orbitrap analyzer
o High-resolution, accurate-mass (HRAM) analysis
o Provide detailed molecular characterization of complex mixtures.
o Includes HESI and APCI ionization sources
Liquid Chromatography (LC): Vanquish Variable Wavelength Detectors
o Vanquish UHPLC system
o Variable Wavelength Detectors
Data Analysis Software: Compound Discoverer 3.4
o A powerful software platform for small-molecule analysis
o Supports diverse workflows for untargeted metabolomics
High-Performance Liquid Chromatography (HPLC):
o Advanced Shimadzu HPLC systems
o Multiple detector types, refractive index (RI), diode array detector (DAD)
Flash and Preparative Chromatography:
o The BUCHI Pure Chromatography System (C-850 Flash/Prep)
o Equipped with UV–Vis detection (200–800 nm) & Evaporative Light Scattering (ELS) detector
Solid Phase Extraction (SPE)
o The department also utilizes a Solid Phase Extraction (SPE) assembly for efficient sample cleanup, concentration, and pre-fractionation prior to analytical or preparative runs. Improves compound recovery and data quality for LC–MS/MS and HPLC applications.
TLCs
o Normal phase silica gel TLCs run
o Reverse phase RP C18 TLCs run